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KMID : 0385020010170030173
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Korean Journal of Laboratory Animal Science
2001 Volume.17 No. 3 p.173 ~ p.177
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Estimation for Enzyme Activity of Energy Metabolism and Subsequent Development of Fresh Bovine Embryos from In Vitro Fertilization
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Kim, Kil Soo
Lee, Eun Ju/Ryoo, Zae Young/Lee, Jeong Woong/Phark, Jun Hong/Choi, Joung Ok/Jung, Boo Kyung
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Abstract
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The studies were conducted to elucidate a relationships between metabolic activities of bovine fertilized eggs and their ability to subsequent development. Bovine embryos were obtained by in vitro fertilization system described elsewhere, except the pretreatment of frozen semen. Enzyme activity of glucose metabolism was calculated in each one of the fresh embryos from 1-cell to blastocyst stages, using enzyme cycling method modified by us for single fresh embryos and they were check for their subsequent development. When subsequent development after measurement was examined using the 4-cell stage embryos, the development rate to the blastocyst stage was not significantly correlated to the hexokinase activity. MDH activity was the highest at the blastocyst stages. MDH activity in the degenerated embryos was significantly lower(P$lt;0.05) as compared with that in normal embryos. MDH activity increased markedly in the presence of glutamate, malate, -ketoglutarate and DNP, indicating that via the TCA cycle that these substrates can be used as energy sources. G6PD activity increased in individual bovine embryos during development from 2-cell to blastocyst stage in the presence or absence of G6P. The results showed that bovine embryos that had lower or higher level than the medium in enzyme activity did not development in co-culture after the measurement of enzyme activity.
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